Recent additions may not be included but will usually appear in 24 hrs or less.
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Effect of <i>Lactobacillus rhamnosus</i> HN001 and <i>Bifidobacterium longum</i> BB536 on the healthy gut microbiota composition at phyla and species level: A preliminary study. β
Go to Publication β
World journal of gastroenterology (World J Gastroenterol ) Vol: 23 Issue 15 Pages: 2696-2704 Pub: 2017 Apr 21 Epub: Authors Toscano M , De Grandi R , Stronati L , De Vecchi E , Drago L , |
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Effect of <i>Lactobacillus rhamnosus</i> HN001 and <i>Bifidobacterium longum</i> BB536 on the healthy gut microbiota composition at phyla and species level: A preliminary study. β
Go to Publication β
World journal of gastroenterology (World J Gastroenterol ) Vol: 23 Issue 15 Pages: 2696-2704 Pub: 2017 Apr 21 Epub: Authors Toscano M , De Grandi R , Stronati L , De Vecchi E , Drago L , |
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Effect of <i>Lactobacillus rhamnosus</i> HN001 and <i>Bifidobacterium longum</i> BB536 on the healthy gut microbiota composition at phyla and species level: A preliminary study. β
Go to Publication β
World journal of gastroenterology (World J Gastroenterol ) Vol: 23 Issue 15 Pages: 2696-2704 Pub: 2017 Apr 21 Epub: Authors Toscano M , De Grandi R , Stronati L , De Vecchi E , Drago L , |
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Effect of <i>Lactobacillus rhamnosus</i> HN001 and <i>Bifidobacterium longum</i> BB536 on the healthy gut microbiota composition at phyla and species level: A preliminary study. β
Go to Publication β
World journal of gastroenterology (World J Gastroenterol ) Vol: 23 Issue 15 Pages: 2696-2704 Pub: 2017 Apr 21 Epub: Authors Toscano M , De Grandi R , Stronati L , De Vecchi E , Drago L , |
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Effect of <i>Lactobacillus rhamnosus</i> HN001 and <i>Bifidobacterium longum</i> BB536 on the healthy gut microbiota composition at phyla and species level: A preliminary study. β
Go to Publication β
World journal of gastroenterology (World J Gastroenterol ) Vol: 23 Issue 15 Pages: 2696-2704 Pub: 2017 Apr 21 Epub: Authors Toscano M , De Grandi R , Stronati L , De Vecchi E , Drago L , |
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The effect of Lactobacillus rhamnosus hsryfm 1301 on the intestinal microbiota of a hyperlipidemic rat model. β
Go to Publication β
BMC complementary and alternative medicine (BMC Complement Altern Med ) Vol: 14 Issue Pages: 386 Pub: 2014 Oct 10 Epub: 2014 Oct 10 Authors Chen D , Yang Z , Chen X , Huang Y , Yin B , Guo F , Zhao H , Zhao T , Qu H , Huang J , Wu Y , Gu R , |
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The effect of Lactobacillus rhamnosus hsryfm 1301 on the intestinal microbiota of a hyperlipidemic rat model. β
Go to Publication β
BMC complementary and alternative medicine (BMC Complement Altern Med ) Vol: 14 Issue Pages: 386 Pub: 2014 Oct 10 Epub: 2014 Oct 10 Authors Chen D , Yang Z , Chen X , Huang Y , Yin B , Guo F , Zhao H , Zhao T , Qu H , Huang J , Wu Y , Gu R , |
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Effect of Lactobacillus rhamnosus hsryfm 1301 on the Gut Microbiota and Lipid Metabolism in Rats Fed a High-Fat Diet. β
Journal of microbiology and biotechnology (J Microbiol Biotechnol ) Vol: 25 Issue 5 Pages: 687-95 Pub: 2015 May Epub: Authors Chen D , Yang Z , Chen X , Huang Y , Yin B , Guo F , Zhao H , Huang J , Wu Y , Gu R , |
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Effect of Lactobacillus rhamnosus hsryfm 1301 on the Gut Microbiota and Lipid Metabolism in Rats Fed a High-Fat Diet. β
Journal of microbiology and biotechnology (J Microbiol Biotechnol ) Vol: 25 Issue 5 Pages: 687-95 Pub: 2015 May Epub: Authors Chen D , Yang Z , Chen X , Huang Y , Yin B , Guo F , Zhao H , Huang J , Wu Y , Gu R , |
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Effect of Lactobacillus rhamnosus hsryfm 1301 on the Gut Microbiota and Lipid Metabolism in Rats Fed a High-Fat Diet. β
Journal of microbiology and biotechnology (J Microbiol Biotechnol ) Vol: 25 Issue 5 Pages: 687-95 Pub: 2015 May Epub: Authors Chen D , Yang Z , Chen X , Huang Y , Yin B , Guo F , Zhao H , Huang J , Wu Y , Gu R , |
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Effect of Lactobacillus rhamnosus hsryfm 1301 on the Gut Microbiota and Lipid Metabolism in Rats Fed a High-Fat Diet. β
Journal of microbiology and biotechnology (J Microbiol Biotechnol ) Vol: 25 Issue 5 Pages: 687-95 Pub: 2015 May Epub: Authors Chen D , Yang Z , Chen X , Huang Y , Yin B , Guo F , Zhao H , Huang J , Wu Y , Gu R , |
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Lactobacillus rhamnosus GG Ameliorated Long-Term Exposure to TiO(2) Nanoparticles Induced Microbiota-Mediated Liver and Colon Inflammation and Fructose-Caused Metabolic Abnormality in Metabolism Syndrome Mice. β
Go to Publication β
Journal of agricultural and food chemistry (J Agric Food Chem ) Vol: 69 Issue 34 Pages: 9788-9799 Pub: 2021 Sep 1 Epub: 2021 Aug 12 Authors Zhao Y , Liu S , Tang Y , You T , Xu H , |
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Lactobacillus rhamnosus GG Ameliorated Long-Term Exposure to TiO(2) Nanoparticles Induced Microbiota-Mediated Liver and Colon Inflammation and Fructose-Caused Metabolic Abnormality in Metabolism Syndrome Mice. β
Go to Publication β
Journal of agricultural and food chemistry (J Agric Food Chem ) Vol: 69 Issue 34 Pages: 9788-9799 Pub: 2021 Sep 1 Epub: 2021 Aug 12 Authors Zhao Y , Liu S , Tang Y , You T , Xu H , |
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Beneficial Shifts in Gut Microbiota by Lacticaseibacillus rhamnosus R0011 and Lactobacillus helveticus R0052 in Alcoholic Hepatitis. β
Go to Publication β
Microorganisms (Microorganisms ) Vol: 10 Issue 7 Pages: Pub: 2022 Jul 21 Epub: 2022 Jul 21 Authors Gupta H , Kim SH , Kim SK , Han SH , Kwon HC , Suk KT , |
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Lacticaseibacillus rhamnosus HN001 alters the microbiota composition in the cecum but not the feces in a piglet model. β
Go to Publication β
Frontiers in nutrition (Front Nutr ) Vol: 9 Issue Pages: 1002369 Pub: 2022 Epub: 2022 Oct 28 Authors Young W , Maclean P , Dunstan K , Ryan L , Peters J , Armstrong K , Anderson R , Dewhurst H , van Gendt M , Dilger RN , Dekker J , Haggarty N , Roy N , |
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A neurotherapeutic approach with Lacticaseibacillus rhamnosus E9 on gut microbiota and intestinal barrier in MPTP-induced mouse model of Parkinson's disease. β
Go to Publication β
Scientific reports (Sci Rep ) Vol: 14 Issue 1 Pages: 15460 Pub: 2024 Jul 4 Epub: 2024 Jul 4 Authors Aktas B , Aslim B , Ozdemir DA , |
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Lacticaseibacillus rhamnosus LRa05 alleviated liver injury in mice with alcoholic fatty liver disease by improving intestinal permeability and balancing gut microbiota. β
Go to Publication β
Beneficial microbes (Benef Microbes ) Vol: 15 Issue 5 Pages: 481-493 Pub: 2024 Jul 3 Epub: 2024 Jul 3 Authors Gu J , Chen Y , Wang J , Gao Y , Gai Z , Zhao Y , Xu F , |
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Fermented Milk Containing Lacticaseibacillus rhamnosus SNU50430 Modulates Immune Responses and Gut Microbiota in Antibiotic-Treated Mice. β
Go to Publication β
Journal of microbiology and biotechnology (J Microbiol Biotechnol ) Vol: 34 Issue 6 Pages: 1299-1306 Pub: 2024 Jun 28 Epub: 2024 Apr 30 Authors Yoon S , Park S , Jung SE , Lee C , Kim WK , Choi ID , Ko G , |
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Dietary supplementation with Lacticaseibacillus rhamnosus IDCC3201 alleviates sarcopenia by modulating the gut microbiota and metabolites in dexamethasone-induced models. β
Go to Publication β
Food & function (Food Funct ) Vol: 15 Issue 9 Pages: 4936-4953 Pub: 2024 May 7 Epub: 2024 May 7 Authors Kang M , Kang M , Yoo J , Lee J , Lee S , Yun B , Song M , Kim JM , Kim HW , Yang J , Kim Y , Oh S , |
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Dietary supplementation with Lacticaseibacillus rhamnosus IDCC3201 alleviates sarcopenia by modulating the gut microbiota and metabolites in dexamethasone-induced models. β
Go to Publication β
Food & function (Food Funct ) Vol: 15 Issue 9 Pages: 4936-4953 Pub: 2024 May 7 Epub: 2024 May 7 Authors Kang M , Kang M , Yoo J , Lee J , Lee S , Yun B , Song M , Kim JM , Kim HW , Yang J , Kim Y , Oh S , |
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Changes in the gut microbiota composition of healthy young volunteers after administration of Lacticaseibacillus rhamnosus LRa05: A placebo-controlled study. β
Go to Publication β
Frontiers in nutrition (Front Nutr ) Vol: 10 Issue Pages: 1105694 Pub: 2023 Epub: 2023 Mar 14 Authors Gai Z , Dong Y , Xu F , Zhang J , Yang Y , Wang Y , |
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Lacticaseibacillus rhamnosus Hao9 exerts antidiabetic effects by regulating gut microbiome, glucagon metabolism, and insulin levels in type 2 diabetic mice. β
Go to Publication β
Frontiers in nutrition (Front Nutr ) Vol: 9 Issue Pages: 1081778 Pub: 2022 Epub: 2023 Jan 5 Authors Han M , Liao W , Dong Y , Bai C , Gai Z , |
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Lacticaseibacillus rhamnosus Hao9 exerts antidiabetic effects by regulating gut microbiome, glucagon metabolism, and insulin levels in type 2 diabetic mice. β
Go to Publication β
Frontiers in nutrition (Front Nutr ) Vol: 9 Issue Pages: 1081778 Pub: 2022 Epub: 2023 Jan 5 Authors Han M , Liao W , Dong Y , Bai C , Gai Z , |
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Lacticaseibacillus rhamnosus Hao9 exerts antidiabetic effects by regulating gut microbiome, glucagon metabolism, and insulin levels in type 2 diabetic mice. β
Go to Publication β
Frontiers in nutrition (Front Nutr ) Vol: 9 Issue Pages: 1081778 Pub: 2022 Epub: 2023 Jan 5 Authors Han M , Liao W , Dong Y , Bai C , Gai Z , |
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Lacticaseibacillus rhamnosus Hao9 exerts antidiabetic effects by regulating gut microbiome, glucagon metabolism, and insulin levels in type 2 diabetic mice. β
Go to Publication β
Frontiers in nutrition (Front Nutr ) Vol: 9 Issue Pages: 1081778 Pub: 2022 Epub: 2023 Jan 5 Authors Han M , Liao W , Dong Y , Bai C , Gai Z , |
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Lacticaseibacillus rhamnosus Probio-M9-Driven Mouse Mammary Tumor-Inhibitory Effect Is Accompanied by Modulation of Host Gut Microbiota, Immunity, and Serum Metabolome. β
Go to Publication β
Nutrients (Nutrients ) Vol: 15 Issue 1 Pages: Pub: 2022 Dec 20 Epub: 2022 Dec 20 Authors Zhang W , Zhang Y , Li Y , Ma D , Zhang H , Kwok LY , |
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Lacticaseibacillus rhamnosus LS8 Ameliorates Azoxymethane/Dextran Sulfate Sodium-Induced Colitis-Associated Tumorigenesis in Mice via Regulating Gut Microbiota and Inhibiting Inflammation. β
Go to Publication β
Probiotics and antimicrobial proteins (Probiotics Antimicrob Proteins ) Vol: 14 Issue 5 Pages: 947-959 Pub: 2022 Oct Epub: 2022 Jul 5 Authors Wang T , Zheng J , Dong S , Ismael M , Shan Y , Wang X , Lü X , |
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Lactobacillus rhamnosus NKU FL1-8 Isolated from Infant Feces Ameliorates the Alcoholic Liver Damage by Regulating the Gut Microbiota and Intestinal Barrier in C57BL/6J Mice. β
Go to Publication β
Nutrients (Nutrients ) Vol: 16 Issue 13 Pages: Pub: 2024 Jul 4 Epub: 2024 Jul 4 Authors Liu H , Fan D , Wang J , Wang Y , Li A , Wu S , Zhang B , Liu J , Wang S , |
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Lactobacillus rhamnosus NKU FL1-8 Isolated from Infant Feces Ameliorates the Alcoholic Liver Damage by Regulating the Gut Microbiota and Intestinal Barrier in C57BL/6J Mice. β
Go to Publication β
Nutrients (Nutrients ) Vol: 16 Issue 13 Pages: Pub: 2024 Jul 4 Epub: 2024 Jul 4 Authors Liu H , Fan D , Wang J , Wang Y , Li A , Wu S , Zhang B , Liu J , Wang S , |
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Lactobacillus rhamnosus Attenuates Cisplatin-Induced Intestinal Mucositis in Mice via Modulating the Gut Microbiota and Improving Intestinal Inflammation. β
Go to Publication β
Pathogens (Basel, Switzerland) (Pathogens ) Vol: 12 Issue 11 Pages: Pub: 2023 Nov 11 Epub: 2023 Nov 11 Authors Alsholi DM , Yacoub GS , Rehman AU , Ullah H , Khan AI , Deng T , Siddiqui NZ , Alioui Y , Farooqui NA , Elkharti M , Li Y , Wang L , Xin Y , |
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Elucidation of Anti-Hypertensive Mechanism by a Novel Lactobacillus rhamnosus AC1 Fermented Soymilk in the Deoxycorticosterone Acetate-Salt Hypertensive Rats. β
Go to Publication β
Nutrients (Nutrients ) Vol: 14 Issue 15 Pages: Pub: 2022 Aug 2 Epub: 2022 Aug 2 Authors Wu H , Jiang L , Shum TF , Chiou J , |
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Elucidation of Anti-Hypertensive Mechanism by a Novel Lactobacillus rhamnosus AC1 Fermented Soymilk in the Deoxycorticosterone Acetate-Salt Hypertensive Rats. β
Go to Publication β
Nutrients (Nutrients ) Vol: 14 Issue 15 Pages: Pub: 2022 Aug 2 Epub: 2022 Aug 2 Authors Wu H , Jiang L , Shum TF , Chiou J , |
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Elucidation of Anti-Hypertensive Mechanism by a Novel Lactobacillus rhamnosus AC1 Fermented Soymilk in the Deoxycorticosterone Acetate-Salt Hypertensive Rats. β
Go to Publication β
Nutrients (Nutrients ) Vol: 14 Issue 15 Pages: Pub: 2022 Aug 2 Epub: 2022 Aug 2 Authors Wu H , Jiang L , Shum TF , Chiou J , |
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Lactobacillus rhamnosus LB1 Alleviates Enterotoxigenic Escherichia coli-Induced Adverse Effects in Piglets by Improving Host Immune Response and Anti-Oxidation Stress and Restoring Intestinal Integrity. β
Go to Publication β
Frontiers in cellular and infection microbiology (Front Cell Infect Microbiol ) Vol: 11 Issue Pages: 724401 Pub: 2021 Epub: 2021 Nov 2 Authors Wu T , Shi Y , Zhang Y , Zhang M , Zhang L , Ma Z , Zhao D , Wang L , Yu H , Hou Y , Gong J , |
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Lactobacillus rhamnosus LB1 Alleviates Enterotoxigenic Escherichia coli-Induced Adverse Effects in Piglets by Improving Host Immune Response and Anti-Oxidation Stress and Restoring Intestinal Integrity. β
Go to Publication β
Frontiers in cellular and infection microbiology (Front Cell Infect Microbiol ) Vol: 11 Issue Pages: 724401 Pub: 2021 Epub: 2021 Nov 2 Authors Wu T , Shi Y , Zhang Y , Zhang M , Zhang L , Ma Z , Zhao D , Wang L , Yu H , Hou Y , Gong J , |
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Lactobacillus rhamnosus from human breast milk ameliorates ulcerative colitis in mice via gut microbiota modulation. β
Go to Publication β
Food & function (Food Funct ) Vol: 12 Issue 11 Pages: 5171-5186 Pub: 2021 Jun 8 Epub: Authors Pang B , Jin H , Liao N , Li J , Jiang C , Shao D , Shi J , |
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Lactobacillus rhamnosus from human breast milk ameliorates ulcerative colitis in mice via gut microbiota modulation. β
Go to Publication β
Food & function (Food Funct ) Vol: 12 Issue 11 Pages: 5171-5186 Pub: 2021 Jun 8 Epub: Authors Pang B , Jin H , Liao N , Li J , Jiang C , Shao D , Shi J , |
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Lactobacillus rhamnosus from human breast milk ameliorates ulcerative colitis in mice via gut microbiota modulation. β
Go to Publication β
Food & function (Food Funct ) Vol: 12 Issue 11 Pages: 5171-5186 Pub: 2021 Jun 8 Epub: Authors Pang B , Jin H , Liao N , Li J , Jiang C , Shao D , Shi J , |
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Lactobacillus rhamnosus from human breast milk ameliorates ulcerative colitis in mice via gut microbiota modulation. β
Go to Publication β
Food & function (Food Funct ) Vol: 12 Issue 11 Pages: 5171-5186 Pub: 2021 Jun 8 Epub: Authors Pang B , Jin H , Liao N , Li J , Jiang C , Shao D , Shi J , |
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Lactobacillus rhamnosus from human breast milk ameliorates ulcerative colitis in mice via gut microbiota modulation. β
Go to Publication β
Food & function (Food Funct ) Vol: 12 Issue 11 Pages: 5171-5186 Pub: 2021 Jun 8 Epub: Authors Pang B , Jin H , Liao N , Li J , Jiang C , Shao D , Shi J , |
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Lactobacillus rhamnosus from human breast milk shows therapeutic function against foodborne infection by multi-drug resistant Escherichia coli in mice. β
Go to Publication β
Food & function (Food Funct ) Vol: 11 Issue 1 Pages: 435-447 Pub: 2020 Jan 29 Epub: Authors Li N , Pang B , Liu G , Zhao X , Xu X , Jiang C , Yang B , Liu Y , Shi J , |
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Probio-M9, a breast milk-originated probiotic, alleviates mastitis and enhances antibiotic efficacy: Insights into the gut-mammary axis. β
Go to Publication β
iMeta (Imeta ) Vol: 3 Issue 4 Pages: e224 Pub: 2024 Aug Epub: 2024 Jul 9 Authors Yu J , Li W , Xu R , Liu X , Gao G , Kwok LY , Chen Y , Sun Z , Liu W , Zhang H , |
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Characterization of the probiotic properties of Lacticaseibacillus rhamnosus LR6 isolated from the vaginas of healthy Korean women against vaginal pathogens. β
Go to Publication β
Frontiers in microbiology (Front Microbiol ) Vol: 14 Issue Pages: 1308293 Pub: 2023 Epub: 2023 Nov 30 Authors Chung Y , Kang SB , Son D , Lee JY , Chung MJ , Lim S , |
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Characterization of the probiotic properties of Lacticaseibacillus rhamnosus LR6 isolated from the vaginas of healthy Korean women against vaginal pathogens. β
Go to Publication β
Frontiers in microbiology (Front Microbiol ) Vol: 14 Issue Pages: 1308293 Pub: 2023 Epub: 2023 Nov 30 Authors Chung Y , Kang SB , Son D , Lee JY , Chung MJ , Lim S , |
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Characterization of the probiotic properties of Lacticaseibacillus rhamnosus LR6 isolated from the vaginas of healthy Korean women against vaginal pathogens. β
Go to Publication β
Frontiers in microbiology (Front Microbiol ) Vol: 14 Issue Pages: 1308293 Pub: 2023 Epub: 2023 Nov 30 Authors Chung Y , Kang SB , Son D , Lee JY , Chung MJ , Lim S , |
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Protective effects of Lacticaseibacillus rhamnosus Hao9 on dextran sulphate sodium-induced ulcerative colitis in mice. β
Go to Publication β
Journal of applied microbiology (J Appl Microbiol ) Vol: 133 Issue 3 Pages: 2039-2049 Pub: 2022 Sep Epub: 2022 Jul 20 Authors Han M , Liao W , Si X , Bai C , Gai Z , |
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Clinical Efficacy of Probiotics for Allergic Rhinitis: Results of an Exploratory Randomized Controlled Trial. β
Go to Publication β
Nutrients (Nutrients ) Vol: 16 Issue 23 Pages: Pub: 2024 Nov 30 Epub: 2024 Nov 30 Authors Lungaro L , Malfa P , Manza F , Costanzini A , Valentini G , Squarzanti DF , Viciani E , Velichevskaya A , Castagnetti A , Barbalinardo M , Gentili D , Cariani A , Ghisellini S , Caputo F , De Giorgio R , Caio G , |
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Lacticaseibacillus rhamnosus LRa05 alleviates cyclophosphamide-induced immunosuppression and intestinal microbiota disorder in mice. β
Go to Publication β
Journal of food science (J Food Sci ) Vol: 89 Issue 12 Pages: 10003-10017 Pub: 2024 Dec Epub: 2024 Nov 26 Authors Zhu M , Yang L , Kong S , Bai Y , Zhao B , |
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Lacticaseibacillus rhamnosus LRa05 alleviates cyclophosphamide-induced immunosuppression and intestinal microbiota disorder in mice. β
Go to Publication β
Journal of food science (J Food Sci ) Vol: 89 Issue 12 Pages: 10003-10017 Pub: 2024 Dec Epub: 2024 Nov 26 Authors Zhu M , Yang L , Kong S , Bai Y , Zhao B , |
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The effect of Lactobacillus rhamnosus on enterohemorrhagic Escherichia coli infection of human intestinal cells in vitro. β
Microbiology and immunology (Microbiol Immunol ) Vol: 47 Issue 6 Pages: 405-9 Pub: 2003 Epub: Authors Hirano J , Yoshida T , Sugiyama T , Koide N , Mori I , Yokochi T , |
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Intravesical Lactobacillus rhamnosus GG Alters Urobiome Composition and Diversity Among People With Neurogenic Lower Urinary Tract Dysfunction. β
Go to Publication β
Topics in spinal cord injury rehabilitation (Top Spinal Cord Inj Rehabil ) Vol: 29 Issue 3 Pages: 44-57 Pub: 2023 Summer Epub: 2023 Aug 16 Authors Groah SL , Rounds AK , Pérez-Losada M , |
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Intravesical Lactobacillus rhamnosus GG Alters Urobiome Composition and Diversity Among People With Neurogenic Lower Urinary Tract Dysfunction. β
Go to Publication β
Topics in spinal cord injury rehabilitation (Top Spinal Cord Inj Rehabil ) Vol: 29 Issue 3 Pages: 44-57 Pub: 2023 Summer Epub: 2023 Aug 16 Authors Groah SL , Rounds AK , Pérez-Losada M , |
All suggestions are computed solely on their predicted microbiome impact. Safety, side-effects etc must be evaluated by your medical professionals before starting. Some items suggests have significant risk of adverse consequences for some people.
Special thanks to David F Morrison and Geert Van Houcke for doing Quality Assurance. Special thanks to Oliver Luk, B.Sc. (Biology) from BiomeSight for spot checking the coding of data from the US National Library of Medicine
This is an Academic site. It generates theoretical models of what may benefit a specific microbiome results.
Copyright 2016-2023 Lassesen Consulting, LLC [2007], DBA, Microbiome Prescription. All rights served.
Permission to data scrap or reverse engineer is explicitly denied to all users. U.S. Code Title 18 PART I CHAPTER 47 Β§β―1030, CETS No.185, CFAA
Use of data on this site is prohibited except under written license. There is no charge for individual personal use. Use for any commercial applications or research requires a written license.
Caveat emptor: Analysis and suggestions are based on modelling (and thus infererence) based on studies. The data sources are usually given for those that wish to consider alternative inferences. theories and models.
Inventions/Methodologies on this site are Patent Pending.
Microbiome Prescription do not make any representations that data or analyses available on this site is suitable for human diagnostic purposes, for informing treatment decisions, or for any other purposes and accept no responsibility or liability whatsoever for such use.
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